Hybridization (or hybridisation) may refer to:

• Hybridization (biology), the process of combining different varieties of organisms to create a hybrid
• Orbital hybridization, in chemistry, the mixing of atomic orbitals into new hybrid orbitals
• Nucleic acid hybridization, the process of joining two complementary strands of nucleic acids - RNA, DNA or oligonucleotides
• In evolutionary algorithms, the merging two or more optimization techniques into a single algorithm
  • Memetic algorithm, a common template for hybridization
• In linguistics, the process of one variety blending with another variety
• The alteration of a vehicle into a hybrid electric vehicle
• In globalization theory, the ongoing blending of cultures
• Hybridization in political election campaign communication, the combining of campaign techniques developed in different countries
• In paleoanthropology, the hypothesis of Neanderthal and human hybridization